Web resources

Database of odorous compounds in water (WaterTOP)

The first Database of Water Odorous Compounds is available online. The database was developed in the frame of WaterTOP (COST Action CA18225). The project was coordinated by Dr. Andreas Dunkel of the Leibniz Institute for Food Systems Biology at the Technical University of Munich (Lebniz-LSB@TUM). It is a fully functional database following the FAIR principles. See the contributors here. The database is complemented by the Water Taste & Odor Wheel (TOW), translated in many languages, a project coordinated by Dr. Ricard Devesa of  the Universitat Politècnica de Catalunya.

WaterTOP training materials and resources

WaterTOP is a European Network on Water Taste & Odor (T&O) that was funded by COST (CA18225) and was coordinated by our group. In the "Products" page of the WaterTOP website you can find training materials, conference presentations, posters and other resources related to water T&O.

Computational MS resources

We find that open and free software and web-based platforms for MS data analysis are more efficient and promote virtual collaborations. We usually convert MS datasets to mzXML/mzML formats using vendor's sofware (e.g., DataAnalysis, Bruker) or MSconvert (ProteoWizard). In this way, we can analyze datasets from various MS instruments and vendors. We pre-process the converted files in MZmine (peak picking, deconvolution, alignment etc). We import the resulting feature tables and the dataset metadata into GNPS/GNPS2 for feature-based molecular networking (FBMN) and library searches. We also import the MS2 spectra file into SIRIUS for de novo molecular formulae annotations, structural elucidation using fragmentation trees and machine learning classification (CANOPUS, ZODIAC, CSI:fingerID). We visualize the combined results in Cytoscape. We use FBMNstats for multivariate statistics and detection of biomarkers. A list of the computational tools we use with associated links and papers can be found here (note that it is updated periodically). A fantastic resource with video trainings is the YouTube channel of the Functional Metabolomics Lab.

Edited books & book chapters

Cyanotoxins in Bloom

Edited by Triantafyllos Kaloudis, Anastasia Hiskia, Theodoros Triantis. April 2022.
Open access. MDPI Books

Water Treatment for Purification from Cyanobacteria and Cyanotoxins

Editor(s):Anastasia E. Hiskia, Theodoros M. Triantis, Maria G. Antoniou, Triantafyllos Kaloudis, Dionysios D. Dionysiou. First published:17 July 2020.
© 2020 John Wiley & Sons Ltd.

Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis.

Editor(s):Jussi Meriluoto, Lisa Spoof, Geoffrey A. Codd.
First published:20 December 2016. DOI:10.1002/9781119068761. © 2017 John Wiley & Sons, Ltd.

Publications (papers)

1. Paraskevopoulou, A.; Kaloudis, T.; Hiskia, A.; Steinhaus, M.; Dimotikali, D.; Triantis, T.M. Volatile Profiling of Spirulina Food Supplements. Foods 2024, 13, 1257. https://doi.org/10.3390/foods13081257

2.     Maria Antonopoulou, Anna Tzamaria, Marta F.F. Pedrosa, Ana R.L. Ribeiro, Adrián M.T. Silva, Triantafyllos Kaloudis, Anastasia Hiskia, Dimitris Vlastos (2024). Spirulina-based carbon materials as adsorbents for drinking water taste and odor control: Removal efficiency and assessment of cyto-genotoxic effects. Science of The Total Environment, Volume 927,172227. https://doi.org/10.1016/j.scitotenv.2024.172227

3.     Dionysiou, D., Blute, N., Kaloudis, T., Weinrich, L. and Zamyadi, A. (2024), Introduction to the topical collection on harmful algal blooms. AWWA Water Science, 6: e1363. https://doi.org/10.1002/aws2.1363

4.     Tim Piel, Giovanni Sandrini, Erik F.J. Weenink, Hongjie Qin, Maria J. van Herk, Mariël Léon Morales-Grooters, J. Merijn Schuurmans, Pieter C. Slot, Geert Wijn, Jasper Arntz, Sevasti-Kiriaki Zervou, Triantafyllos Kaloudis, Anastasia Hiskia, Jef Huisman, Petra M. Visser (2024). Shifts in phytoplankton and zooplankton communities in three cyanobacteria-dominated lakes after treatment with hydrogen peroxide. Harmful Algae, Volume 133, 102585, https://doi.org/10.1016/j.hal.2024.102585 .

5.     Robert Konkel, Aleksandra Milewska, Nguyen Dan Thuc Do, Emilia Barreto Duran, Artur Szczepanski, Jacek Plewka, Ewa Wieczerzak, Sofia Iliakopoulou, Triantafyllos Kaloudis, Dirk Jochmans, Johan Neyts, Krzysztof Pyrc, Hanna Mazur-Marzec (2023). Anti-SARS-CoV-2 activity of cyanopeptolins produced by Nostoc edaphicum CCNP1411, Antiviral Research 219, 105731, https://doi.org/10.1016/j.antiviral.2023.105731

6.     Konkel, R.; Cegłowska, M.; Szubert, K.; Wieczerzak, E.; Iliakopoulou, S.; Kaloudis, T.; Mazur-Marzec, H. Structural Diversity and Biological Activity of Cyanopeptolins Produced by Nostoc edaphicum CCNP1411. Marine Drugs 2023, 21, 508. https://doi.org/10.3390/md21100508

7.     Reyhan Akcaalan, Ricard Devesa-Garriga, Andrea Dietrich, Martin Steinhaus, Andreas Dunkel, Veronika Mall, Maura Manganelli, Simona Scardala, Emanuela Testai, Geoffrey A. Codd, Frantisek Kozisek, Maria Antonopoulou, Ana Rita Lado Ribeiro, Maria José Sampaio, Anastasia Hiskia, Theodoros M. Triantis, Dionysios D. Dionysiou, Gianluca Li Puma, Linda Lawton, Christine Edwards, Henrik Rasmus Andersen, Despo Fatta-Kassinos, Popi Karaolia, Audrey Combès, Kristel Panksep, Sevasti-Kiriaki Zervou, Meriç Albay, Latife Köker, Ekaterina Chernova, Sofia Iliakopoulou, Elisabeth Varga, Petra M. Visser, Angelika Ioanna Gialleli, Zuhal Zengin, Nikos Deftereos, Phani Miskaki, Christophoros Christophoridis, Aikaterina Paraskevopoulou, Tsair-Fuh Lin, Arash Zamyadi, Galina Dimova and Triantafyllos Kaloudis (2022), Water taste and odor (T&O): Challenges, gaps and solutions from a perspective of the WaterTOP network. Chemical Engineering Journal Advances, Volume 12, 100409, https://doi.org/10.1016/j.ceja.2022.100409

8.     Fabio Varriale, Luciana Tartaglione, Sevasti-Kiriaki Zervou, Christopher O. Miles, Hanna Mazur-Marzec, Theodoros M. Triantis, Triantafyllos Kaloudis, Anastasia Hiskia, Carmela Dell’Aversano (2022). Untargeted and targeted LC-MS and data processing workflow for the comprehensive analysis of oligopeptides from cyanobacteria. Chemosphere, 137012, https://doi.org/10.1016/j.chemosphere.2022.137012

9. Kaloudis T.; Hiskia, A.; Triantis, T.M. Cyanotoxins in Bloom: Ever-Increasing Occurrence and Global Distribution of Freshwater Cyanotoxins from Planktic and Benthic Cyanobacteria. Toxins 2022, 14, 264. https://doi.org/10.3390/toxins14040264

10.   Zervou, S.-K.; Kaloudis, T.; Gkelis, S.; Hiskia, A.; Mazur-Marzec, H. Anabaenopeptins from Cyanobacteria in Freshwater Bodies of Greece. Toxins 2022, 14, 4. https://doi.org/10.3390/toxins14010004

11.   Donis, D., Mantzouki, E…., Kaloudis, T.,…., Ibelings, B.W. (2021). Stratification strength and light climate explain variation in chlorophyll a at the continental scale in a European multilake survey in a heatwave summer. Limnol Oceanogr. https://doi.org/10.1002/lno.11963

12.     C. Christophoridis, C.J. Pestana, T. Kaloudis, L.A. Lawton, T.M. Triantis, A. Hiskia (2021). Radiolytic degradation of 2-methylisoborneol and geosmin in water: Reactive radical species and transformation pathways. Chemical Engineering Journal Advances, Volume 8, 100196, https://doi.org/10.1016/j.ceja.2021.100196

13.     Hammoud, N.A.; Zervou, S.-K.; Kaloudis, T.; Christophoridis, C.; Paraskevopoulou, A.; Triantis, T.M.; Slim, K.; Szpunar, J.; Fadel, A.; Lobinski, R.; Hiskia A. (2021). Investigation of the Occurrence of Cyanotoxins in Lake Karaoun (Lebanon) by Mass Spectrometry, Bioassays and Molecular Methods. Toxins 2021, 13, 716. https://doi.org/10.3390/toxins13100716

14.     Zervou, S.-K.; Moschandreou, K.; Paraskevopoulou, A.; Christophoridis, C.; Grigoriadou, E.; Kaloudis, T.; Triantis, T.M.; Tsiaoussi, V.; Hiskia, A. (2021). Cyanobacterial Toxins and Peptides in Lake Vegoritis, Greece. Toxins 2021, 13, 394. https://doi.org/10.3390/toxins13060394

15.     Maria Antonopoulou, Nikolaos Ioannidis, Triantafyllos Kaloudis, Theodoros M. Triantis, Anastasia Hiskia (2020). Kinetic and mechanistic investigation of water taste and odor compound 2-isopropyl-3-methoxy pyrazine degradation using UV-A/Chlorine process. Science of The Total Environment 732, 138404, https://doi.org/10.1016/j.scitotenv.2020.138404

16.     Sevasti – Kiriaki Zervou, Triantafyllos Kaloudis, Anastasia Hiskia, Hanna Mazur-Marzec (2020). Fragmentation mass spectra dataset of linear cyanopeptides – microginins. Data in Brief 31, 105825, https://doi.org/10.1016/j.dib.2020.105825

17.     Sevasti - Kiriaki Zervou, Spyros Gkelis, Triantafyllos Kaloudis, Anastasia Hiskia, Hanna Mazur-Marzec (2020). New microginins from cyanobacteria of Greek freshwaters. Chemosphere 248, 125961, https://doi.org/10.1016/j.chemosphere.2020.125961

18.     Gkelis, S., Panou, M., Konstantinou, D., Apostolidis, P., Kasampali, A., Papadimitriou, S., Kati, D., Di Lorenzo, G.M., Ioakeim, S., Zervou, S.-K., Christophoridis, C., Triantis, T.M., Kaloudis, T., Hiskia, A., Arsenakis, M. (2019). Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece. Toxins 2019, 11, 436. https://doi.org/10.3390/toxins11080436

19.       Korina Manolidi, Theodoros M. Triantis, Triantafyllos Kaloudis, Anastasia Hiskia (2019). Neurotoxin BMAA and its isomeric amino acids in cyanobacteria and cyanobacteria-based food supplements. Journal of Hazardous Materials 365,  346-365. https://doi.org/10.1016/j.jhazmat.2018.10.084  

20.       Christophoridis, C., Zervou, S.-K., Manolidi, K., Katsiapi, M., Moustaka-Gouni, M., Kaloudis, T., Triantis, T.M., Hiskia, A. (2018). Occurrence and diversity of cyanotoxins in Greek lakes. Scientific Reports 8, 17877. https://doi.org/10.1038/s41598-018-35428-x

21.     Evanthia Mantzouki et al. (2018). A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins. Scientific Data 5, 180226. https://doi.org/10.1038/sdata.2018.226 .

Data citation: Mantzouki, E. et al. (2018). Environmental Data Initiative https://doi.org/10.6073/pasta/dabc352040fa58284f78883fa9debe37

22.     Arevik Minasyan, Christophoros Christophoridis, Alan E. Wilson, Sevasti-Kiriaki Zervou, Triantafyllos Kaloudis, Anastasia Hiskia (2018). Diversity of cyanobacteria and the presence of cyanotoxins in the epilimnion of Lake Yerevan (Armenia). Toxicon 150, 28-38. https://doi.org/10.1016/j.toxicon.2018.04.021

23.     Manthos Panou, Sevasti-Kiriaki Zervou, Triantafyllos Kaloudis, Anastasia Hiskia, Spyros Gkelis (2018). A Greek Cylindrospermopsis raciborskii strain: Missing link in tropic invader’s phylogeography tale. Harmful Algae 80, 96-106. https://doi.org/10.1016/j.hal.2018.10.002

24.     Evanthia Mantzouki, Miquel Lurling, Jutta Fastner et al. (2018). Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins. Toxins 10(4), 156. https://doi.org/10.3390/toxins10040156

25.   Kaloudis, T., Meriluoto, J., & Blaha, L. (2017). Foreword to the Themed Issue “Cyanobacteria”. Advances in Oceanography and Limnology, 8(1). https://doi.org/10.4081/aiol.2017.6674

26.   Meriluoto, J., Blaha, L., Bojadzija, G., Bormans, M., Brient, L., Codd, G. A., Drobac, D., Faassen, E. J., Fastner, J., Hiskia, A., Ibelings, B. W., Kaloudis, T., Kokocinski, M., Kurmayer, R., Pantelić, D., Quesada, A., Salmaso, N., Tokodi, N., Triantis, T. M., Visser, P. M., & Svirčev, Z. (2017). Toxic cyanobacteria and cyanotoxins in European waters – recent progress achieved through the CYANOCOST Action and challenges for further research. Advances in Oceanography and Limnology, 8(1). https://doi.org/10.4081/aiol.2017.6429

27.     Gkelis, S., Panou, M., Chronis, I., Zervou, S.-K., Christophoridis, C., Manolidi, K., Ntislidou, C., Triantis, T. M., Kaloudis, T., Hiskia, A., Kagalou, I., & Lazaridou, M. (2017). Monitoring a newly re-born patient: water quality and cyanotoxin occurrence in a reconstructed shallow Mediterranean lake. Advances in Oceanography and Limnology, 8(1). https://doi.org/10.4081/aiol.2017.6350

28.     Zervou, S.-K., Christophoridis, C., Kaloudis, T., Triantis, T. M., & Hiskia, A. (2017). New SPE-LC-MS/MS method for simultaneous determination of multi-class cyanobacterial and algal toxins. Journal of Hazardous Materials, 323(Part A), 56-66. https://doi.org/10.1016/j.jhazmat.2016.07.020 

29.     Moustaka-Gouni, M., Hiskia, A., Genitsaris, S., Katsiapi, M., Manolidi, K., Zervou, S.-K., Christophoridis, C., Triantis, T. M., Kaloudis, T., & Orfanidis, S. (2017). First report of Aphanizomenon favaloroi occurrence in Europe associated with saxitoxins and a massive fish kill in Lake Vistonis, Greece. Marine and Freshwater Research, 68(4), 793-800. https://doi.org/10.1071/MF16029

30.     Faassen, E. J., Antoniou, M. G., Beekman-Lukassen, W., Blahova, L., Chernova, E., Christophoridis, C., Combes, A., Edwards, C., Fastner, J., Harmsen, J., Hiskia, A., Ilag, L. L., Kaloudis, T., Lopicic, S., Lürling, M., Mazur-Marzec, H., Meriluoto, J., Porojan, C., Viner-Mozzini, Y., & Zguna, N. (2016). A collaborative evaluation of LC-MS/MS based methods for BMAA analysis: Soluble bound BMAA found to be an important fraction. Marine Drugs, 14(3). http://dx.doi.org/10.3390/md14030045

31.       Fotiou, T., Triantis, T. M., Kaloudis, T., O'Shea, K. E., Dionysiou, D. D., & Hiskia, A. (2016). Assessment of the roles of reactive oxygen species in the UV and visible light photocatalytic degradation of cyanotoxins and water taste and odor compounds using C–TiO2. Water Research, 90 (Supplement C), 52-61. https://doi.org/10.1016/j.watres.2015.12.006

32.       He, X., de la Cruz, A. A., Hiskia, A., Kaloudis, T., O'Shea, K., & Dionysiou, D. D. (2015). Destruction of microcystins (cyanotoxins) by UV-254nm-based direct photolysis and advanced oxidation processes (AOPs): Influence of variable amino acids on the degradation kinetics and reaction mechanisms. Water Research, 74, 227-238. https://doi.org/10.1016/j.watres.2015.02.011

33.       Fotiou, T., Triantis, T., Kaloudis, T., & Hiskia, A. (2015). Photocatalytic degradation of cylindrospermopsin under UV-A, solar and visible light using TiO2. Mineralization and intermediate products. Chemosphere, 119(Supplement), S89-S94. https://doi.org/10.1016/j.chemosphere.2014.04.045

34.       Fotiou, T., Triantis, T. M., Kaloudis, T., & Hiskia, A. (2015). Evaluation of the photocatalytic activity of TiO2 based catalysts for the degradation and mineralization of cyanobacterial toxins and water off-odor compounds under UV-A, solar and visible light. Chemical Engineering Journal, 261 (Supplement C), 17-26. https://doi.org/10.1016/j.cej.2014.03.095

35.       Fotiou, T., Triantis, T. M., Kaloudis, T., Papaconstantinou, E., & Hiskia, A. (2014). Photocatalytic degradation of water taste and odour compounds in the presence of polyoxometalates and TiO2: Intermediates and degradation pathways. Journal of Photochemistry and Photobiology A: Chemistry, 286 (Supplement C), 1-9. https://doi.org/10.1016/j.jphotochem.2014.04.013

36.       Kaloudis, T., Zervou, S.-K., Tsimeli, K., Triantis, T. M., Fotiou, T., & Hiskia, A. (2013). Determination of microcystins and nodularin (cyanobacterial toxins) in water by LC–MS/MS. Monitoring of Lake Marathonas, a water reservoir of Athens, Greece. Journal of Hazardous Materials, 263(Part 1), 105-115. https://doi.org/10.1016/j.jhazmat.2013.07.036

37.       Fotiou, T., Triantis, T. M., Kaloudis, T., Pastrana-Martínez, L. M., Likodimos, V., Falaras, P., Silva, A. M. T., & Hiskia, A. (2013). Photocatalytic Degradation of Microcystin-LR and Off-Odor Compounds in Water under UV-A and Solar Light with a Nanostructured Photocatalyst Based on Reduced Graphene Oxide–TiO2 Composite. Identification of Intermediate Products. Industrial & Engineering Chemistry Research, 52(39), 13991-14000. http://dx.doi.org/10.1021/ie400382r

38.       de la Cruz, A. A., Hiskia, A., Kaloudis, T., Chernoff, N., Hill, D., Antoniou, M. G., He, X., Loftin, K., O'Shea, K., Zhao, C., Pelaez, M., Han, C., Lynch, T. J., & Dionysiou, D. D. (2013). A review on cylindrospermopsin: the global occurrence, detection, toxicity and degradation of a potent cyanotoxin. Environmental Science: Processes & Impacts, 15(11), 1979-2003. http://dx.doi.org/10.1039/C3EM00353A

39.       He, X., Pelaez, M., Westrick, J. A., O’Shea, K. E., Hiskia, A., Triantis, T., Kaloudis, T., Stefan, M. I., de la Cruz, A. A., & Dionysiou, D. D. (2012). Efficient removal of microcystin-LR by UV-C/H2O2 in synthetic and natural water samples. Water Research, 46(5), 1501-1510. https://doi.org/10.1016/j.watres.2011.11.009

40.     Sharma, V. K., Triantis, T. M., Antoniou, M. G., He, X., Pelaez, M., Han, C., Song, W., O’Shea, K. E., de la Cruz, A. A., Kaloudis, T., Hiskia, A., & Dionysiou, D. D. (2012). Destruction of microcystins by conventional and advanced oxidation processes: A review. Separation and Purification Technology, 91(Supplement C), 3-17. https://doi.org/10.1016/j.seppur.2012.02.018

41.     Triantis, T. M., Fotiou, T., Kaloudis, T., Kontos, A. G., Falaras, P., Dionysiou, D. D., Pelaez, M., & Hiskia, A. (2012). Photocatalytic degradation and mineralization of microcystin-LR under UV-A, solar and visible light using nanostructured nitrogen doped TiO2. Journal of Hazardous Materials, 211-212(Supplement C), 196-202. https://doi.org/10.1016/j.jhazmat.2011.11.042

42.   Triantis, T., Tsimeli, K., Kaloudis, T., Thanassoulias, N., Lytras, E., & Hiskia, A. (2010). Development of an integrated laboratory system for the monitoring of cyanotoxins in surface and drinking waters. Toxicon, 55(5), 979-989. https://doi.org/10.1016/j.toxicon.2009.07.012

43.     Dimitrakopoulos, I. K., Kaloudis, T. S., Hiskia, A. E., Thomaidis, N. S., & Koupparis, M. A. (2010). Development of a fast and selective method for the sensitive determination of anatoxin-a in lake waters using liquid chromatography–tandem mass spectrometry and phenylalanine-d 5 as internal standard. Analytical and Bioanalytical Chemistry, 397(6), 2245-2252. https://doi.org/10.1007/s00216-010-3727-3

44. Farmaki, E., Kaloudis, T., Dimitrou, K., Thanasoulias, N., Kousouris, L., & Tzoumerkas, F. (2007). Validation of a FT-IR method for the determination of oils and grease in water using tetrachloroethylene as the extraction solvent. Desalination, 210(1), 52-60. https://doi.org/10.1016/j.desal.2006.05.032